After the events of Rise of the Crystal Gems, Mojo Jojo has assembled the most dangerous criminals in New Townsville and New York City to get rid of the Powerpuff Girls and the Crystal Gems once and for all, including a new threat named Carnage who will do nothing to kill the Gems and the Girls for vengeance. Now, Team Powerpuff and the Crystal Gems will have to team-up with Spider-Man and a famous cat-and-mouse duo to take down the sinister plot of Mojo and Carnage.
- This will focus somewhere between The Week of Sardonyx and the Out of the World event.
- The sequel will have new gems like Ruby, Sapphire, Peridot, Lapis, and more from the Steven Universe world who weren't shown in the previous film, as well as Team Powerpuff's new threat, Carnage.
- The music will be from Lorne Balfe and John Debney while the additional music will be from Heitor Pereira and Aivi & Surasshu.
- This features new story elements from the two Lego spin offs, The Lego Batman Movie and The Lego Ninjago Movie with the Spider-Man Trilogy and the Spider Man TAS episodes, Venom Returns and Carnage, both of which feature Carnage.